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Diagnostic Kit for Antibodies to Chicken infectious bronchitis virus (ELISA)
Product Introduction
Intended usePrinciple of the assayMaterials provided with the kitStorage

Chicken infectious bronchitis virus (IBV) antibody test kit is used to test the antibody of IBV in chicken serum. It can evaluate the IBV vaccine immunization status in poultry farming and auxiliary diagnosis of infected chicken by serology.

This kit utilizes the principles of indirect ELISA. It is comprised of microplate coated with Chicken IBV. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to IBV, the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme- conjugated second antibody is added into the washed microplate and it will specifically bind with the antibody/antigen complex on the microplate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate   12mL×1
  3.   10×Concentrated wash solution   50mL×1
  4.   Chromogen solution A   6mL×1
  5.   Chromogen solution B   6mL×1
  6.   Sample diluent   50mL×1
  7.   Stop solution   6mL×1
  8.   Positive control(blue cap, inactivated)   0.8mL×1
  9.   Negative control(blue cap, inactivated)   0.8mL×1
  10.   Serologecal Plate   1
  11.   Plate Sealer   2
  12.   Instruction   1

stored at 2~8℃

      If you need the manual for more details, you can click here
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