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Diagnostic Kit for Antibodies to Porcine Pseudorabies disease virus (ELISA)
Product Introduction
Intended usePrinciple of the assayMaterials provided with the kitStorage

This kit is used to test the antibody of porcine pseudorabies virus (PRV) in pig’s serum. It can also be used to evaluate the vaccine immunization status of PRV and apply to serodiagnosis of infected pigs. But this kit cannot be used to distinguish the difference between vaccine immunization and infection by wild virus.

This kit is comprised of microplate coated with porcine PRV antigen, enzyme-conjugated second antibody and other reagents. It utilizes the principles of ELISA to detect antibody specific to PRV in pig’s serum and plasma. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to PRV, the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme- conjugated second antibody is added into the washed microplate and it will specifically bind with the antibody/antigen complex on the microplate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm and proportional to the concentration of specific antibody.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate (red cap)   11mL×1
  3.   Sample diluent (yellow cap)   25mL×1
  4.   20×Concentrated wash solution(transparent cap)   25mL×1
  5.   Chromogen solution A(white cap)   6mL×1
  6.   Chromogen solution B(black cap)   6mL×1
  7.   Stop solution(yellow cap)   6mL×1
  8.   Positive control(red cap, inactivated)   0.5mL×1
  9.   Negative control(green cap, inactivated)   0.5mL×1
  10.   Plate Sealer   1
  11.   Instruction   1

The kit is stored at 2~8℃. Do not freeze.

      If you need the manual for more details, you can click here
  Region   Name   Email
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  South America   Gwyneth
  Asia   Britney
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  Western & Middle Europe   Perry

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