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Diagnostic Kit for Antibodies to Porcine Epidemic Diarrhea Virus (ELISA)
Product Introduction
Intended usePrinciple of the assayMaterials provided with the kitStorage

Porcine epidemic diarrhea is a highly contagious intestinal tract disease of swine caused by porcine epidemic diarrhea virus (PEDV). It is characterized by vomiting, dehydration and loss of appetite. Pigs in all ages are prone to be infected.This kit is used to test the antibody of PEDV (PEDV-Ab) in pig’s serum and plasma. It can also be used to evaluate the vaccine immunization status of PEDV.

This kit is comprised of microplate coated with PEDV recombinant N protein, enzyme-conjugated second antibody and other reagents. It utilizes the principles of ELISA to detect antibody specific to PEDV in pig’s serum and plasma. Control serum and diluted serum samples are added into the microplate during experiment. After incubation, if the sample contains antibody specific to PEDV, the antibody will bind with the antigen coated in the microplate. The microplate should be washed with wash solution to remove unbound antibody and other components. The enzyme- conjugated second antibody is added into the washed microplate and it will specifically bind with the antibody/antigen complex on the microplate. The microplate should be washed once again to remove unbound enzyme conjugate. Then TMB substrate solution is added into wells to react with enzyme and appears blue in color. The enzyme-substrate reaction is terminated by the addition of the stop solution, the color changes from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm and proportional to the concentration of specific antibody.

  1.   Micro ELISA Plate   96T×1
  2.   Enzyme Conjugate (red cap)   11mL×1
  3.   Sample diluent (blue cap)   30mL×1
  4.   20×Concentrated wash solution   40mL×1
  5.   Chromogen solution A(white cap)   7mL×1
  6.   Chromogen solution B(black cap)   7mL×1
  7.   Stop solution(yellow cap)   7mL×1
  8.   Positive control(red cap)   1mL×1
  9.   Negative control(blue cap)   1mL×1
  10.   Plate Sealer   1
  11.   Instruction   1

The kit is stored at 2~8℃. Do not freeze.

      If you need the manual for more details, you can click here
  Region   Name   Email
  North America   John
  South America   Gwyneth
  Asia   Britney
  Japan & Taiwan   Yumi
  South Asia & Australia   Hanny
  Eastern & Northern Europe   Cathy
  Southern Europe & Africa   Lucy
  Western & Middle Europe   Perry

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