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SPlink Detection Kit
Product Introduction
ComponentsDescriptionAssay ProcedureProduct Storage  Application&Size
Reagent(colourless  liquid)3%H2O2  deionized water
Reagent A(blue fluid)normal goat serum for blocking
Reagent B(yellow fluid)biotinylated second antibody working solution
Reagent C(orange fluid)HRP  labeled streptavidin working solution(SA-HRP)

Streptavidin is a protein that has similar binding properties to avidin.It is isolated from streptomyces avidinii. Streptavidin has a molecular weight of 60 KD and has 4 subunits. Each subunit can bind one molecule of biotin.SPlink Detection Kit is coupled steptavidin with horseradish peroxidase (HRP). This highly sensitive detector conjugate binds to biotinylated secondary antibodies in immunohistochemistry.

1. Section paraffin,dewax conventionally

    2. Antigen retrieval(this step is optional according to the experiment requirement)

    3. Incubate with 3%H2O2 deionized water for 5~10min to eliminate endogenous peroxidase activity.

    Wash with PBS,3min/time for 3 times

    4. Add reagent A, incubate at room temperature for 10~15min, abandon the reagent,don’t wash

    5. Add primary antibody with proper dilution ratio,incubate at 37℃ for 2~3h or at 4℃ for one night

    6. Wash with PBS,3min/time for 3 times

    7. Add reagent B,incubate at 37℃ or room temperature for 10~15min

    8. Wash with PBS,3min/time for 3 times

    9. Add reagent C,incubate at 37℃ or room temperature for 10~15min

    10. Wash with PBS,3min/time for 3 times

    11. Operate chromogenic reaction with chromogenic reagent(DAB or ACE)

    12. Wash with tap water

    13. Make the section counterstained,dewatered, transparent if the experiment requires

    14. Mount the section with proper mounting medium

Stored at 2°C - 8°C. Shading light.

 Application: IHC

 Size: 3 mL/6 mL/18 mL

 If you need the manual for more details, you can click here
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